Abstract :

ABSTRACT

Background: Selecting the most suitable Acellularized Dermal Matrix (ADM) for clinical applications requires careful consideration of various factors, including differences in manufacturing processes and potential immune responses. Both in-vitro and in-vivo tests are essential for evaluating the performance of ADMs and predicting their behavior post-implantation.

Methods: In this study, ADMs derived from human and porcine sources were implanted into mouse skin to assess macrophage reactions using RAW264.7 cells as a model system.

Results: The histological analysis revealed that human ADM induced a higher degree of reaction and fibrosis in mouse skin compared to porcine ADM at 2 weeks post-operation. However, these differences became less pronounced and similar between the two groups at 14 weeks post-operation. Furthermore, human ADM elicited a greater upregulation of pro-inflammatory genes in macrophages at 24 hours post-stimulation compared to porcine ADM.

Conclusion: The findings suggest that macrophage responses to ADMs could serve as predictive indicators of in-vivo responses. Understanding these responses may aid in the selection of appropriate ADMs for specific clinical applications.

Keywords: Acellularized Dermal Matrix; Human; Porcine; Fibrosis; Wound.