Asada Leelahavanichkul*

Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand

Correspondence to: Asada Leelahavanichkul, Department of Microbiology, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand. E-mail: alelahavanit@gmail.com

Received: May 07, 2023; Accepted: May 25, 2023; Published: June 04, 2023

Citation: Leelahavanichkul A. Macrophage Reactions to Acellularized Dermal Matrix: A Comparative Study Between Human and Porcine Sources and Their Implications for In Vivo Fibrosis. J Immunol Microbiol. 2023;1(1):18-21.

Copyright: © 2023 Leelahavanichkul A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

ABSTRACT

Background: Selecting the most suitable Acellularized Dermal Matrix (ADM) for clinical applications requires careful consideration of various factors, including differences in manufacturing processes and potential immune responses. Both in-vitro and in-vivo tests are essential for evaluating the performance of ADMs and predicting their behavior post-implantation.

Methods: In this study, ADMs derived from human and porcine sources were implanted into mouse skin to assess macrophage reactions using RAW264.7 cells as a model system.

Results: The histological analysis revealed that human ADM induced a higher degree of reaction and fibrosis in mouse skin compared to porcine ADM at 2 weeks post-operation. However, these differences became less pronounced and similar between the two groups at 14 weeks post-operation. Furthermore, human ADM elicited a greater upregulation of pro-inflammatory genes in macrophages at 24 hours post-stimulation compared to porcine ADM.

Conclusion: The findings suggest that macrophage responses to ADMs could serve as predictive indicators of in-vivo responses. Understanding these responses may aid in the selection of appropriate ADMs for specific clinical applications.

Keywords: Acellularized Dermal Matrix; Human; Porcine; Fibrosis; Wound.