Abstract :

ABSTRACT

The objective of this study was to characterize antibiotic resistant Enterobacteriaceae present in wastewater discharged into the Mindoube Municipal Landfill.

Sampling was conducted on September 29, 2022, with 12 samples collected from the stream point of tanker truck in the landfill to the river. Bacteriological analysis at the IRET Microbiology Laboratory revealed the isolation of one bacterial colony per sample, with identified species including Escherichia coli (58.3%), Serratia Ficaria/Pantoea spp (47.1%), Klebsiella oxytoca (33.3%), and Klebsiella pneumoniae (25%). Antibiotic susceptibility testing indicated high resistance rates to Ceftazidime and Cefepime (100%), moderate resistance to Cefotaxime (54%), and significant resistance to Amoxicillin and Clavulanic Acid (75%). Resistance to sulphonamides, quinolones, and aminoglycosides was observed at lower levels. These findings suggest that the wastewater from the Mindoube landfill is a significant source of contamination for the nearby stream. Consequently, the proximity of the landfill and the polluted river poses health risks to residents who rely on the river water for daily household activities. The presence of multi-resistant bacteria in the water raises concerns about potential therapeutic failures in treating infectious diseases in individuals exposed to this environment.

Keywords: Mindoube Municipal Landfill; Wastewater; Enterobacteriaceae; Antibiotic Resistance.