Antibiotic Resistance of Enterobacteriaceae in Wastewater Samples from the Mindoube Municipal Landfill, Libreville, Gabon
Pierre Philippe Mbehang Nguema1*, Yvon-Bert Pambou1, Emelie Arlette Apinda Legnouo1, Roméo Wenscesla Lendamba2
1Institut de Recherche en Écologie Tropicale, Centre National de la Recherche Scientifique et Technologique (IRET/CENAREST), Microbiology Laboratory, PB: 13345 Quartier Gros-bouquet, Libreville, Gabon
2Centre de Recherches Médicales de Lambaréné (CERMEL), Department of Clinical Operations, PB: 242, Lambaréné, Gabon
Correspondence to: Pierre Philippe Mbehang Nguema, Institut de Recherche en Écologie Tropicale, Centre National de la Recherche Scientifique et Technologique (IRET/CENAREST), Microbiology Laboratory, PB: 13345 Quartier Gros-bouquet, Libreville, Gabon. E-mail: mbehangphlippe@gmail.com
Received: March 27, 2023; Accepted: April 14, 2023; Published: April 23, 2023
Citation: Nguema PPM, Pambou YB, Legnouo EAA, Lendamba RW. Antibiotic Resistance of Enterobacteriaceae in Wastewater Samples from the Mindoube Municipal Landfill, Libreville, Gabon. J Immunol Microbiol. 2023;1(1):11-15.
Copyright: © 2023 Nguema PPM. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
ABSTRACT
The objective of this study was to characterize antibiotic resistant Enterobacteriaceae present in wastewater discharged into the Mindoube Municipal Landfill.
Sampling was conducted on September 29, 2022, with 12 samples collected from the stream point of tanker truck in the landfill to the river. Bacteriological analysis at the IRET Microbiology Laboratory revealed the isolation of one bacterial colony per sample, with identified species including Escherichia coli (58.3%), Serratia Ficaria/Pantoea spp (47.1%), Klebsiella oxytoca (33.3%), and Klebsiella pneumoniae (25%). Antibiotic susceptibility testing indicated high resistance rates to Ceftazidime and Cefepime (100%), moderate resistance to Cefotaxime (54%), and significant resistance to Amoxicillin and Clavulanic Acid (75%). Resistance to sulphonamides, quinolones, and aminoglycosides was observed at lower levels. These findings suggest that the wastewater from the Mindoube landfill is a significant source of contamination for the nearby stream. Consequently, the proximity of the landfill and the polluted river poses health risks to residents who rely on the river water for daily household activities. The presence of multi-resistant bacteria in the water raises concerns about potential therapeutic failures in treating infectious diseases in individuals exposed to this environment.
Keywords: Mindoube Municipal Landfill; Wastewater; Enterobacteriaceae; Antibiotic Resistance.
